ABSTRACT
Abstract Background Evidence suggests that monoclonal B-cell lymphocytosis precedes all chronic lymphocytic leukemia cases, although the molecular mechanisms responsible for disease progression are not understood. Aberrant miRNA expression may contribute to the pathogenesis of chronic lymphocytic leukemia. The objective of this study was to compare miRNA expression profiles of patients with Binet A chronic lymphocytic leukemia with those of subjects with high-count monoclonal B-cell lymphocytosis and healthy volunteers (controls). Methods Twenty-one chronic lymphocytic leukemia patients, 12 subjects with monoclonal B-cell lymphocytosis and ten healthy volunteers were enrolled in this study. Flow cytometry CD19+CD5+-based cell sorting was performed for the chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis groups and CD19+ cells were sorted to analyze the control group. The expressions of miRNAs (miR-15a, miR-16-1, miR-29b, miR-34a, miR-181a, miR-181b and miR-155) were determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Results Significant differences between the expressions in the chronic lymphocytic leukemia and monoclonal B-cell lymphocytosis groups were restricted to the expression of miR-155, which was higher in the former group. A comparison between healthy controls and monoclonal B-cell lymphocytosis/chronic lymphocytic leukemia patients revealed higher miR-155 and miR-34a levels and lower miR-15a, miR-16-1, miR-181a and miR-181b in the latter group. Conclusions Our results show a progressive increase of miR-155 expression from controls to monoclonal B-cell lymphocytosis to chronic lymphocytic leukemia. The role of miR-155 in the development of overt chronic lymphocytic leukemia in individuals with monoclonal B-cell lymphocytosis must be further analyzed.
Subject(s)
Humans , Stanford-Binet Test , B-Lymphocytes , Leukemia, Lymphocytic, Chronic, B-Cell , MicroRNAs , LymphocytosisABSTRACT
BACKGROUND: Monoclonal B-cell lymphocytosis is classified as 'high-count or clinical' monoclonal B-cell lymphocytosis and 'low-count or population' monoclonal B-cell lymphocytosis. Previously, 167 first-degree relatives pertaining to sporadic (non-familial) chronic lymphocytic leukemia families were studied and the presence of seven monoclonal B-cell lymphocytosis individuals was reported.OBJECTIVE: The aim of this report is to describe the outcomes of five of the original monoclonal B-cell lymphocytosis individuals.METHODS: Flow cytometry analysis was performed on mononuclear cells previously isolated from peripheral blood samples. A strategy of sequential gating designed to identify the population of CD19+/CD5+ B-lymphocytes was used and, subsequently, the monoclonal B-cell lymphocytosis cells were characterized by the CD20weak/CD79bweak/negative phenotype.RESULTS: The monoclonal B-cell lymphocytosis clone showed consistent stability over time with little variations in size. After a median follow-up of 7.6 years, none of the five monoclonal B-cell lymphocytosis individuals progressed to chronic lymphocytic leukemia or other B-cell lymphoproliferative disease.CONCLUSIONS: The data of this study suggest that chronic lymphocytic leukemia-like monoclonal B-cell lymphocytosis detected in the context of sporadic chronic lymphocytic leukemia families is not prone to clinical evolution and could be just a sign of immune senescence.
Subject(s)
Humans , B-Lymphocytes , Leukemia, B-Cell , Leukemia, Lymphocytic, Chronic, B-Cell , Family Relations/ethnology , Flow Cytometry , Lymphocytosis , Lymphoproliferative Disorders , Antibodies, MonoclonalABSTRACT
A violência na infância e na adolescência é um grave fenômeno social e de saúde e não pode continuar sendo vista como uma responsabilidade policial. O Estatuto da Criança e do Adolescente reserva aos profissionais de saúde a atuação nos diagnósticos de maus tratos e os procedimentos com a notificação. O não cumprimento dessa responsabilidade ocorre devido à falta de conhecimento da lei ou por não estarem convencidos de que devem exercer esse papel. É importante afirmar que a notificação não é um ato pessoal, mas uma obrigação legal. Diante de uma vítima, o médico precisa seguir algumas normas que, por vezes, geram conflitos entre os preceitos legais e éticos. Na categoria médica é consensual comunicar as autoridades, sempre que se tratarem de menores de 14 anos e alguns casos de adolescentes. A evidência de maus tratos constitui justa causa para a quebra do sigilo. O paciente deve ser informado da possibilidade de um exame pericial no Instituto Médico Legal, capaz de uma avaliação mais criteriosa. Além da violência estrutural devido às desigualdades sociais existentes em nosso meio, as crianças e adolescentes também são violentadas dentro dos seus lares. Todas as formas de violência podem causar danos ao desenvolvimento biopsicossocial em curto, médio e longo prazos
The violence in childhood and adolescence is a serious social and health problem and it must not continue to be seen as the police's responsibility. The Statute of the Child and Adolescent assigns to health care professionals the action regarding diagnoses of injuries and procedures towards the notification. The unfulfillment of this responsibility is due to the lack of knowledge of the law or because they are not convinced that they must have this role. It is important to state that the notification is not a personal act, but a legal obligation. Facing the victim, the doctor must follow certain rules that sometimes conflict between legal and ethical ideas. In the medical it is consensual to communicate the authorities when the person is less than 14 years old and in some cases of adolescents. The evidence of mistreatment constitutes legal cause for confidentiality breach. The patient must be informed of the possibility of going through a peer review, in the Institute of Legal Medicine, that is capable of a more careful evaluation. In addition to the structural violence because of the social inequalities in our country, children and adolescents are also damaged inside their houses. All forms of violence can cause damage to the biopsychosocial development in short, medium and long terms
Subject(s)
Humans , Child , Adolescent , Child Abuse/diagnosis , Child Abuse/legislation & jurisprudence , Child Abuse/psychology , Mandatory Reporting , Health Personnel/ethics , Sex Offenses , Domestic Violence/ethics , Domestic Violence/legislation & jurisprudence , Domestic Violence/psychology , Child Behavior/psychology , Adolescent Behavior/psychology , Socioeconomic FactorsABSTRACT
OBJETIVOS: descrever aspectos da assistência e resultados obstétricos da gravidez em adolescentes atendidas em um centro de atendimento terciário do Ceará e comparar os resultados maternos e perinatais entre adolescentes precoces e tardias. MÉTODOS: em estudo transversal, analítico, avaliaram-se 2.058 casos, sendo 322 (15,6 por cento) de adolescentes precoces e 1.736 (84,4 por cento) tardias, atendidas no ano de 2000. Foram analisados as intercorrências clínicas no pré-natal, tipo de parto, indicações de cesárea, idade gestacional no parto, peso do recém-nascido ao nascimento, adequação do peso à idade gestacional, índices de Apgar no primeiro e quinto minuto de vida, presença de malformações e óbito neonatal. Utilizaram-se o teste exato de Fisher e o chi2 na comparação entre os dois grupos. Calculou-se também a razão de prevalência. RESULTADOS: do total de partos ocorridos no período, 25,9 por cento eram de adolescentes e a média de idade destas foi de 17,2 anos. Constatou-se que 88 por cento freqüentaram o pré-natal, sendo 60 por cento com número insuficiente de consultas. As intercorrências clínicas mais freqüentes foram a pré-eclâmpsia (14,7 por cento), a anemia (12,9 por cento) e a infecção do trato urinário (6,4 por cento), sem diferença de freqüência entre os grupos. Ocorreram 31,3 por cento de nascimentos por cesárea, sendo a pré-eclâmpsia a principal indicação nas duas faixas etárias (25 e 23 por cento, respectivamente). A freqüência de Apgar menor que 7 no primeiro minuto foi de 19,9 por cento no grupo das adolescentes precoces e 14,2 por cento entre as tardias (x²=6,96, p=0,008). Não houve diferença quanto à freqüência de prematuridade (20,2 vs 16,1 por cento), recém-nascido pequeno para idade gestacional (12,4 vs 10,4 por cento), baixos escores de Apgar de quinto minuto (5,3 vs 3,3 por cento), malformações congênitas (3,1 vs 2,7 por cento) e morte neonatal (1,6 vs 3,1 por cento). CONCLUSÕES: as gestantes adolescentes...
PURPOSE: to describe the obstetric outcomes in pregnant adolescents at a tertiary hospital and to compare the maternal and labor outcomes between precocious and late adolescents. METHODS: in a transversal analytical study, 2058 cases were evaluated, considering 322 (15.65 percent) from the precocious group and 1736 (84.35 percent) from the late group that delivered at the "Maternidade Escola Assis Chateaubriand/UFC" from January 1, 2000 to December 31, 2000. The clinical complications in the prenatal period, kind of delivery, indications for cesarean section, birth gestational age at birth, birth weight, comparison of birth weight and gestational age, Apgar score at the first and fifth minute, presence of malformations, and neonatal death were analyzed. The exact Fisher and the chi2 tests were used to compare both groups. The prevalence ratio was calculated. RESULTS: from of total of deliveries, 25.95 percent belonged to adolescents. The average age was 17.19 years. Prenatal visits were made by 88 percent of the patients, but 60 percent had an insufficient number of visits. The most frequent clinical situations were preeclampsia (14.72 percent), anemia (12.97 percent) and urinary tract infections (6.37 percent), with no statistical difference between the groups. Thirty-one and three percent of the births were by cesarean section, preeclampsia being the main indication in the two age groups (25 and 23 percent, respectively). The frequency of an Apgar score less than 7 at the first minute was 19,9 percent in the precocious adolescent group and 14,2 percent in the late adolescent group (x²=6,96, p=0.008). There was no statistical difference regarding prematurity rate (20.2 vs 16.1 percent), low-birth weight infants (12.4 vs 10.4 percent), low Apgar score at the fifth minute (5.3 vs 3.3 percent), congenital malformations (3.1 vs 2.7 percent), and neonatal death (5.3 vs 3.3 percent). CONCLUSIONS: the precocious and late pregnant adolescents...
Subject(s)
Pregnancy , Adolescent , Humans , Female , Perinatal Care , Pre-Eclampsia , Pregnancy Complications , Pregnancy in Adolescence , Pregnancy OutcomeABSTRACT
CONTEXT AND OBJECTIVE: Spontaneous bacterial peritonitis (SBP) is a frequent and severe complication of cirrhotic patients with ascites. It has been proposed that the reagent strip for leukocyte esterase designed for the testing of urine (Combur test® UX) could be a useful tool for diagnosing SPB. The aim of this study was to assess the sensitivity and specificity of urine test strips for diagnosing SBP in cirrhotic patients with ascites. DESIGN AND SETTING: Prospective study, at a university hospital in northeastern Brazil. METHODS: Forty-two unselected consecutive cirrhotic patients (32 males; mean age: 51.7 ± years) were included, and a total of 100 paracenteses were performed. All ascitic fluid samples were analyzed using the reagent strip and cytology, neutrophils, lymphocyte count, appropriate biochemical tests and culturing. The strips were considered positive if the color became purple on a colorimetric scale. RESULTS: Nine patients were diagnosed with SBP using cytology (> 250 neutrophils/mm³), and the strips were positive for all these nine patients with SBP. In one sample, the strip was positive but the neutrophil count was less than 250 cells/mm³. For 86 samples, both the strips and cytology were negative. At the threshold of 250 neutrophils/mm³ in ascitic fluid, the sensitivity, specificity, positive predictive value and negative predictive value for the strips were respectively 100 percent, 98.9 percent, 92.3 percent and 100 percent. CONCLUSION: The Combur test® UX urine screening test is a very sensitive and specific method for diagnosing SBP in cirrhotic patients with ascites.
CONTEXTO E OBJETIVO: Peritonite bacteriana espontânea (PBE) é uma freqüente e grave complicação em pacientes cirróticos com ascite. Vem sendo proposto o uso de fitas reagente para esterase leucocitária, utilizadas para diagnóstico de infecção urinária, como instrumento para diagnosticar PBE. O objetivo deste estudo é avaliar a sensibilidade e a especificidade da fita reagente Combur Test® UX no diagnóstico de PBE em pacientes cirróticos com ascite. TIPO DE ESTUDO E LOCAL: Estudo prospectivo, realizado no Hospital Universitário Walter Cantídio, Fortaleza, Ceará, Brasil. MÉTODOS: Quarenta e dois pacientes consecutivos, não selecionados (32 homens, com média de idade de 51,7 ± anos) foram incluídos e um total de 100 paracenteses foram realizadas. O líquido ascítico foi avaliado pela fita reagente (Combur Test® UX), citologia, contagem de neutrófilos, linfócitos, testes bioquímicos e cultura. As fitas reagentes foram consideradas positivas se havia mudança na cor para roxo na escala colorimétrica fornecida pelo fabricante. RESULTADOS: Foram diagnosticados nove pacientes com PBE pela citologia (> 250 neutrófilos/mm³), estando a fita Combur Test® UX positiva em todos os nove pacientes. Em uma amostra o teste da fita foi positivo, embora a contagem de neutrófilos fosse < 250 células/mm³. Nas outras 86 amostras ambos os testes foram negativos. Tendo-se como ponto de corte 250 PMN/mm³ no líquido ascítico, a sensibilidade, a especificidade, o valor preditivo positivo e o valor preditivo negativo foram respectivamente 100 por cento, 98,9 por cento, 92,3 por cento e 100 por cento. CONCLUSÕES: Combur Test® UX (fita reagente para urinanálise) é um método sensível e específico para o diagnóstico de PBE em pacientes cirróticos com ascite.